The 2nd International CTNNB1 Syndrome Conference was held on the 14th and 15th of June 2024 in Ljubljana, Slovenia. This event marks an important milestone for our community as we recently successfully completed preclinical studies and have initiated our manufacturing process. Over the conference days, we have put together a program with the main focus on the gene replacement therapy, clinical readiness and updates on other treatment solution programs. As many of the symptom-related topics were already covered last year, this year we wanted to focus particularly on the rarer of the rare, including heart defects and epilepsy.